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返回產(chǎn)品中心>沃特世蛋白質(zhì)反相色譜柱是一種基于蛋白質(zhì)在溶液中的相對(duì)疏水性的分離技術(shù)，分析時(shí)使用裝填有短鏈鍵合相的大孔徑顆粒（例如，300Å）的色譜柱。如果使用離子對(duì)試劑（例如0.1% TFA）來(lái)zui大限度減少不良離子干擾，通常利用逐漸升高的有機(jī)溶劑濃度梯度來(lái)控制分離。一般來(lái)說(shuō)，洗脫順序取決于蛋白質(zhì)或蛋白質(zhì)亞基的疏水性，疏水性zui弱的蛋白質(zhì)將首先洗脫出來(lái)。
沃特世蛋白質(zhì)反相色譜柱 介紹：
蛋白質(zhì)反相色譜柱
蛋白質(zhì)反相色譜分析是一種基于蛋白質(zhì)在溶液中的相對(duì)疏水性的分離技術(shù)，分析時(shí)使用裝填有短鏈鍵合相的大孔徑顆粒（例如，300Å）的色譜柱。如果使用離子對(duì)試劑（例如0.1% TFA）來(lái)zui大限度減少不良離子干擾，通常利用逐漸升高的有機(jī)溶劑濃度梯度來(lái)控制分離。一般來(lái)說(shuō)，洗脫順序取決于蛋白質(zhì)或蛋白質(zhì)亞基的疏水性，疏水性zui弱的蛋白質(zhì)將首先洗脫出來(lái)。此外，顆粒組成（硅膠vs雜化顆粒）、孔徑、鍵合相類(lèi)型和密度，以及分離條件（例如梯度持續(xù)時(shí)間、分離溫度、流速）等等對(duì)于實(shí)現(xiàn)滿足應(yīng)用要求的分離而言也非常重要。
沃特世蛋白質(zhì)反相色譜柱部分色譜柱：
| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 300 µm X 100 mm, 1/pkg 部件代碼: 186007568 | ---- |
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| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 300 µm X 50 mm, 1/pkg 部件代碼: 186007567 | ---- |
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| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 300 µm X 150 mm, 1/pkg 部件代碼: 186007569 | ---- |
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| DeltaPak C4 Guard Column, 300Å, 5 µm, 3.9 mm X 20 mm, 2/pkg 部件代碼: WAT046885 | ---- | |||
| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 150 µm X 100 mm, 1/pkg 部件代碼: 186007495 | ---- |
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| DeltaPak C4 Column, 300Å, 15 µm, 3.9 mm X 300 mm, 1/pkg 部件代碼: WAT011812 | ---- | |||
| DeltaPak C4 Guard-Pak Insert, 300Å, 5 µm, 10/pkg 部件代碼: WAT036865 | ---- |
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| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 75 µm X 100 mm, 1/pkg 部件代碼: 186007493 | ---- |
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| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 100 µm X 100 mm, 1/pkg 部件代碼: 186007494 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 50 mm, 1/pkg 部件代碼: 186005589 | ---- |
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| ACQUITY UPLC Protein BEH C4 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg 部件代碼: 186004899 | ---- |
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| ACQUITY UPLC Protein BEH C4 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg 部件代碼: 186006549 | ---- | |||
| BioSuite Phenyl Column, 1000Å, 10 µm, 2 mm X 75 mm, 1/pkg 部件代碼: 186002156 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 100 mm, 1/pkg 部件代碼: 186005590 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 150 mm, 1/pkg 部件代碼: 186005591 | ---- |
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| DeltaPak C4 Column, 300Å, 5 µm, 3.9 mm X 150 mm, 1/pkg 部件代碼: WAT011794 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 150 mm, 1/pkg 部件代碼: 186004497 | ---- |
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| ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 3/pkg 部件代碼: 186004623 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 100 mm, 1/pkg 部件代碼: 186004496 | ---- |
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| DeltaPak C4 Column, 100Å, 5 µm, 3.9 mm X 150 mm, 1/pkg 部件代碼: WAT011796 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 50 mm, 1/pkg 部件代碼: 186004495 | ---- |
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| DeltaPak C4 Column, 100Å, 15 µm, 3.9 mm X 300 mm, 1/pkg 部件代碼: WAT011807 | ---- |
沃特世蛋白質(zhì)反相色譜柱
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